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Further supporting this concept, we observed loss of IL 2 induced GM CSF production on protein and messenger RNA levels upon specific STAT5 inhibition18 in TH cells (Fig. 4c e). GM CSF production induced by endogenous IL 2 was similarly reduced by STAT5 inhibition.Figure 4: IL 2 induces GM CSF via phosphorylation of STAT5.(a,b) Sorted naive TH cells (CD4+CD45ROCD25/medCD127+) were activated (CD3) in the presence of the indicated cytokine for 5 days.