The quality of the RNA may be affected by time taken to obtain the tissue biopsy until it is frozen, and the time to cut the sections to freeze in the OCT blocks used for LCMEC. Comparison between mammary gland epithelial cells before and after laser microdissection was performed (Figure 2). Alveolar (acinar) structures (Figure 2A) lined by mammary epithelial cells (yellow arrows) could be easily distinguished after staining mammary tissue sections with Mayer’s hematoxylin (Sigma).
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No amplification was noted in any of the coexisting DH lesions.Another interesting finding was that the amplification ratios were related to the stage of the lesion, with the highest ratio in the invasive component. For 21 cases in which there was concordance between invasive cancers and DCIS, the average amplification ratio for the invasive component was 6.2, and for the DCIS, it was 5.2. For those 13 cases, having the whole spectrum of benign and malignant lesions, the average amplification ratios for DH, ADH, DCIS, and invasive cancers were 1.0, 4.1, 5.7, and 6.6, respectively (Table 3).